All rights reserved. Tracking dye for nucleic acid or protein electrophoresis. J Colloid Interface Sci. Bromphenol Blue - Compound Summary. Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 22; 540: 148-154. Please contact us to place your order, or try again later. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Nanocellulose for gel electrophoresis. We're doing our best to keep everyone healthy and safe in the workplace while also avoiding the interruptions to our day-to-day operations. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.[4]. Room temperature in continental US; may vary elsewhere. Mendoza L, et, al. We do not sell to patients. Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol), Concentration (start) × Volume (start) = Concentration (final) × Volume (final), This equation is commonly abbreviated as: C1V1 = C2V2, Bromophenol blueOtherstrackingdyeelectrophoresispHindicatorInhibitorinhibitorinhibit. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6[1][2]. Mendoza L, et, al. Santa Cruz Biotechnology, Inc. Bromophenol Blue (CAS 115-39-9) Properties. Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Your information is safe with us. Dangui AZ, et, al. As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. Please note: All products are "FOR RESEARCH USE ONLY. Thanks, your subscription has been confirmed. Please store the product under the recommended conditions in the Certificate of Analysis. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. We have received your request and will respond to you as soon as possible. Publishing research using ab146339? O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O. Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of about 300 base pairs, in 2% agarose as 150 bp. © 1998-2020 Abcam plc. PubChem Compound. Bromphenol Blue - Use and Manufacturing. Submission failed, please try again later. Please let us know so that we can cite the reference in this datasheet. Get resources and offers direct to your inbox. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. As the situation with COVID-19 continues to unfold in every community, MedChemExpress is responding to the uncertainty caused by this outbreak thoughtfully and cautiously. Store under desiccating conditions. We do not sell to patients. All of the co-solvents are provided by MCE. Need more advice on solubility, usage and handling? Bromophenol blue is also used as a dye. Preferential solvation bromophenol blue in water-alcohol binary mixture. The product can be stored for up to 12 months. Sorry, but the email address you supplied was invalid. Migrates at approximately the same rate as 300-500bp DNA… Bromophenol blue, Tracking dye (CAS 115-39-9) (ab146339) | Abcam Bromophenol blue is structurally related to phenolphthalein (a popular indicator). There are currently no Customer reviews or Questions for ab146339.Please use the links above to contact us or submit feedback about this product. To our knowledge, customised protocols are not required for this product. * Required Fields. Migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. Your need for high quality reagent doesn't stop during difficult times, and neither do we. If you need to change the delivery plan for items ordered, please contact us via email [email protected]. Dangui AZ, et, al. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. [1]. [7] Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index. Based on 1 publication(s) in Google Scholar. Solutions of the dye, therefore, are blue. Please try the standard protocols listed below and let us know how you get on. PubChem Compound. Agonists, activators, antagonists and inhibitors. Our Cookie Policy explains how you can opt-out of the cookies we use. pH indicator (pH 3.0 (yellow) - 4.6 (blue)).,,, "Agarose gel electrophoresis (basic method)", "Quantification of dichromatism: a characteristic of color in transparent materials",, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 October 2020, at 16:16. Store at Room Temperature. This phenomenon is called dichromatic color. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Reaxys. At neutral pH, the dye absorbs red light most strongly and transmits blue light. J Colloid Interface Sci. Please visit our frequently asked questions (FAQ) page for more details. 2018 Oct 5; 203: 333-341. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed.

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